TITLE : Development of a Real - Time PCR method for the identification
نویسندگان
چکیده
A Real Time-PCR method based on TaqMan technology for the 14 identification of Scomber Scombrus has been developed. A system of specific 15 primers and a Minor Groove Binding (MGB) TaqMan probe based on 16 sequences of the mitochondrial cytochrome b region was designed. The 17 method was successfully tested in 81 specimens of Scomber scombrus and 18 related species and validated in 26 different commercial samples. An average 19 Threshold cycle (Ct) value of 15.3 was obtained with Scomber scombrus DNA. 20 With the other species tested fluorescence signal was not detected or Ct was 21 significantly higher (P<0.001). The efficiency of the assay was estimated to be 22 92.41%, with 100% specificity, and no cross reactivity was detected with any 23 other species. These results reveal that the developed method is a rapid and 24 efficient tool to unequivocally identify S. scombrus and may aid in the 25 prevention of fraud or mislabeling in mackerel products. 26
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